Sequence Analysis of the Spodoptera litura Multicapsid Nucleopolyhedrovirus Genome
نویسندگان
چکیده
منابع مشابه
Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome.
The nucleotide sequence of the DNA genome of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV), a group II NPV, was determined and analysed. The genome contains 135611 bp and has a G+C content of 44 mol%. Computer-assisted analysis revealed 139 ORFs of 150 nucleotides or larger; 103 have homologues in Autographa californica MNPV (AcMNPV) and a further 16 have homologues in other bacul...
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The ecdysteroid UDP-glucosyltransferase gene (egt) of Spodoptera littoralis multicapsid nucleopolyhedrovirus (SpliMNPV) is a homologue of the Autographa californica MNPV (AcMNPV) egt gene, which has been found to block insect moulting. Infection of larvae with an egt-deleted AcMNPV resulted in enhanced mortality as compared to infection with the wild-type virus. Consequently, deletion of an egt...
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Spodoptera litura (S. litura) is one of the most destructive agricultural pests worldwide. There is urgent need for a nuclear polyhedrosis virus that is specific to S. litura. To date, there have been no reports regarding the responses of S. litura cells to early Spodoptera litura nucleopolyhedrovirus (SpltNPV) infection due to the lack of a reference genome and transcriptome for S. litura. In ...
متن کاملSequence and transcriptional analysis of the ubiquitin gene cluster in the genome of Spodoptera exigua nucleopolyhedrovirus.
The nucleotide sequence of a 1200 bp DNA fragment of Spodoptera exigua nucleopolyhedrovirus (SeMNPV) was determined. This sequence contained a cluster of two open reding frames (ORFs), one coding for a viral ubiquitin (v-ubi) and another with homology to orf2 of Autographa californica (Ac) MNPV and Orgyia pseudotsugata (Op) MNPV. The vubi ORF is 240 nucleotides (nt) long, potentially encoding a...
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A continuous cell line, designated KU-SENL-1 (SENL1), has been established from minced neonate larvae of Spodoptera exigua (Lepidoptera: Noctuidae) treated with collagenase. The primary culture was maintained in TC100 medium supplemented with 10% Fetal Bovine Serum (FBS), 3% Helicoverpa armigera hemolymph and incubated at 27°C. This continuous cell line was cultured in TC100 medium supplemented...
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ژورنال
عنوان ژورنال: Virology
سال: 2001
ISSN: 0042-6822
DOI: 10.1006/viro.2001.1056